restriction mappings การใช้

• This restriction mapping sets up a fundamental correspondence:
• For example the most common application of restriction mapping is presented : Determining the orientation of a cloned insert.
• A "'restriction map "'is a map of known restriction sites within a sequence of DNA . Restriction mapping requires the use of restriction enzymes.
• Restriction mapping, Southern analysis, and DNA sequencing permitted identification of five recombinant phage-containing inserts which, when overlapped at common sequences, coded the entire 35kb Factor IX gene.
• Restriction mapping is a very useful technique when used for determining the orientation of an insert in a cloning vector, by mapping the position of an off-center restriction site in the insert.
• The study of genomic DNA by restriction mapping, polymerase chain reaction analysis and DNA sequencing showed the gene being 11.5 kilobases in length, consisting of five exons separated by four introns.